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Fig. 3 | Gut Pathogens

Fig. 3

From: The O-antigen negative ∆wbaV mutant of Salmonella enterica serovar Enteritidis shows adaptive resistance to antimicrobial peptides and elicits colitis in streptomycin pretreated mouse model

Fig. 3

Attachment and invasion of S. Enteritidis wild-type and its isogenic mutants to HCT116 cell line. a HCT116 cells were seeded at the density of 2 × 105 cells per well and bacterial strains were infected at the MOI of 10. Infection was performed on ice followed by incubation at 4 °C for 30 min. Non adherent bacteria were removed by washing and number of adhering bacteria was enumerated by plating serial dilution. b For invasion, HCT116 cells were seeded at the density of 2 × 105 cells per well and bacterial strains were infected at the MOI of 10. Number of invading bacteria was determined through standard gentamicin protection assay. SPI1 negative strain ΔinvC served as an experimental control. Experiments were performed in triplicates. Percentage of adhesion, invasion and uptake of mutant was compared to wild-type (normalized to 100). Error bar indicate the standard deviation of three independent experiments. Statistical analysis was performed using t test. The percentage of adhesion and invasion replication of Δprt and Δtyv was compared with that of ΔwbaV and level of significance is indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001), ns not significant

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