Fig. 4
From: Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells
Comparison of EPEC or EHEC O157:H7 invasion into HT-29, HT-29-OBN, HT-29-Gal, and HT-29-Gal-OBN cells using bacteria invasion assay. HT-29, HT-29-OBN, HT-29-Gal, and HT-29-Gal-OBN cells were incubated with EPEC or EHEC O157:H7, the adherent bacteria were removed by gentamicin, and the internalized EPEC or EHEC O157:H7 within the different cells was quantified by determining the CFU following by the plating of serial dilutions of the bacteria on MacConkey agar with overnight incubation at 37 °C. Asterisk represents a significant difference (p < 0.05), double asterisk represents a highly significant difference (p < 0.01)