Non-Typhoidal Salmonella in poultry meat and diarrhoeic patients: prevalence, antibiogram, virulotyping, molecular detection and sequencing of class I integrons in multidrug resistant strains

Table 4 Primers’ sequences and amplification’s conditions used for detection of Salmonella virulence genes

Target gene	Primers’ sequences 5′–3′	Amplified segment (bp)	Primary denaturation	Amplification (35 cycles)			Final extension	References
Target gene	Primers’ sequences 5′–3′	Amplified segment (bp)	Primary denaturation	Secondary denaturation	Annealing	Extension	Final extension	References
pefA	TGTTTCCGGGCTTGTGCT	700	94 °C 10 min	94 °C 45 s	55 °C 45 s	72 °C 45 s	72 °C 10 min	[4]
pefA	CAGGGCATTTGCTGATTCTTCC	700	94 °C 10 min	94 °C 45 s	55 °C 45 s	72 °C 45 s	72 °C 10 min	[4]
hilA	CGGAAGCTTATTTGCGCCATGCT GAGGTAG	854	94 °C 10 min	94 °C 45 s	60 °C 45 s	72 °C 45 s	72 °C 10 min	[37]
hilA	GCATGGATCCCCGCCGGCGAGAT TGTG	854	94 °C 10 min	94 °C 45 s	60 °C 45 s	72 °C 45 s	72 °C 10 min	[37]
sopB	TCAGAAGRCGTCTAACCACTC	517	94 °C 5 min	94 °C 30 s	58 °C 30 s	72 °C 30 s	72 °C 7 min	[38]
sopB	TACCGTCCTCATGCACACTC	517	94 °C 5 min	94 °C 30 s	58 °C 30 s	72 °C 30 s	72 °C 7 min	[38]
stn	TTGTGTCGCTATCACTGGCAACC	617	94 °C 10 min	94 °C 45 s	59 °C 45 s	72 °C 45 s	72 °C 10 min	[4]
stn	ATTCGTAACCCGCTCTCGTCC	617	94 °C 10 min	94 °C 45 s	59 °C 45 s	72 °C 45 s	72 °C 10 min	[4]

ISSN: 1757-4749