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Table 3 H2O2 production and IC50 values of human and vole Lactobacillus strains for bovine bile, taurocholate, and HgCl2

From: Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster)

Strain

H2O a2

Bovine bile (%)b

Taurocholate (mmol/L)b

HgCl2 (mmol/L)b

24 h

48 h

PV010

−

<0.13

7 ± 0.3

0.1 ± 0.03

0.1 ± 0.00

PV011

−

3.0 ± 0.9

>14

0.1 ± 0.01

0.1 ± 0.01

PV012

−

4.2 ± 0.3

>14

0.2 ± 0.01

0.2 ± 0.04

PV013

−

1.6 ± 0.1

>14

0.1 ± 0.03

0.1 ± 0.03

PV014

−

1.8 ± 0.8

>14

0.1 ± 0.03

0.1 ± 0.03

PV015

−

0.8 ± 0.4

>14

0.1 ± 0.03

0.2 ± 0.23

PV016

−

<0.13

7 ± 1.2

0.2 ± 0.16

0.2 ± 0.23

PV017

−

2.8 ± 0.6

>14

0.2 ± 0.07

0.2 ± 0.10

PV018

−

3.3 ± 0.7

>14

0.2 ± 0.07

0.2 ± 0.11

PV019

−

3.6 ± 0.7

>14

0.1 ± 0.03

0.2 ± 0.13

PV020

−

<0.13

>14

0.2 ± 0.22

0.2 ± 0.22

PV021

−

0.4 ± 0.3

14

0.1 ± 0.10

0.2 ± 0.22

PV022

−

0.2 ± 0.1

9 ± 2.3

0.2 ± 0.06

0.2 ± 0.24

PV023

−

0.2 ± 0.1

10 ± 3.5

0.1 ± 0.12

0.2 ± 0.15

PV024

−

1.4 ± 0.5

14

0.1 ± 0.05

0.1 ± 0.09

PV025

+

<0.13

5 ± 1.2

0.1 ± 0.05

0.2 ± 0.23

PV026

−

<0.13

8 ± 1.2

0.2 ± 0.27

0.2 ± 0.25

PV027

−

<0.13

8 ± 1.7

0.1 ± 0.11

0.2 ± 0.12

PV028

−

<0.13

9 ± 0.3

0.1 ± 0.09

0.2 ± 0.23

PV029

−

<0.13

9 ± 1.7

0.2 ± 0.25

0.2 ± 0.25

PV030

+

0.2 ± 0.1

10 ± 2

0.2 ± 0.25

0.2 ± 0.26

PV031

−

<0.13

9 ± 0.7

0.1 ± 0.08

0.4 ± 0.23

PV032

−

0.13 ± 0.1

3 ± 1.6

0.3 ± 0.20

0.4 ± 0.12

PV033

−

0.2 ± 0.1

13 ± 1.2

0.2 ± 0.18

0.2 ± 0.10

PV034

+

0.2 ± 0.1

10 ± 1.2

0.1 ± 0.08

0.2 ± 0.11

PV035

−

0.3 ± 0.2

9 ± 1.0

0.3 ± 0.16

0.3 ± 0.12

PV036

−

<0.13

8 ± 3.9

0.1 ± 0.04

0.1 ± 0.05

PV037

+

0.2 ± 0.1

10 ± 2.3

0.1 ± 0.02

0.4 ± 0.07

PV038

−

<0.13

11 ± 2.6

0.2 ± 0.14

0.2 ± 0.11

PV039

−

0.8 ± 0.2

>14

0.1 ± 0.03

0.2 ± 0.05

Lj

+

4.0 ± 0.8

>14

0.02 ± 0.0

0.02 ± 0.0

LGG

−

1.0 ± 0.1

>14

0.1 ± 0.04

0.2 ± 0.11

RC14

+

n. d.

n. d.

n. d.

n. d.

  1. aPurple color indicator for hydrogen peroxide production visible (+) or not visible (−) around colonies on ABTS agar
  2. bIC50 values shown indicate the concentration of inhibitor (mean of triplicate experiments ± standard deviations) that led to 50 % growth reduction. For out of-out-range values the upper or lower concentration limits tested are shown. L. johnsonii ATCC 33200 (Lj), RC14, and LGG are included as reference strains