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Fig. 4 | Gut Pathogens

Fig. 4

From: De Novo sphingolipid synthesis is essential for Salmonella-induced autophagy and human beta-defensin 2 expression in intestinal epithelial cells

Fig. 4

Involvement of NOD2 in the suppressive effect of myriocin on Salmonella-induced hBD-2 expression in SW480 cells. a Western blots to confirm knockdown of NOD2 in SW480 cells. b SW480 cells were transfected with control siRNA or NOD2 siRNA (siRNA = non-target control siRNA; siNOD2 = siRNA to NOD2) for 48 h. The transfected cells were left uninfected (CON) or infected for 1 h with S. typhimurium wild-type strain SL1344. c SW480 cells were infected by S. typhimurium wild-type strain SL1344 in the absence (methanol as vehicle) or presence of myriocin. Total RNA was prepared and analyzed by real-time quantitative PCR to estimate amounts of hBD-2 transcript. The amount of hBD-2 mRNA expression, normalized to the corresponding amount of GAPDH transcript, is shown as the fold increase over uninfected, control cells. Results are represented as mean ± S.E.M. for at least three determinations from independent experiments. An asterisk indicates a significant difference (p < 0.05)

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Gut Pathogens

ISSN: 1757-4749