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Fig. 5 | Gut Pathogens

Fig. 5

From: De Novo sphingolipid synthesis is essential for Salmonella-induced autophagy and human beta-defensin 2 expression in intestinal epithelial cells

Fig. 5

Effect of myriocin on Salmonella-activated intracellular signals in SW480 cells. SW480 cells were left untreated (CON), or treated with 10 µM myriocin, and then infected with wild-type S. Typhimurium strain SL1344 for the times indicated. Activation of the Akt in cell membrane fraction and ERK and JNK in cytosolic fraction were analyzed by immunoblotting with antibodies to phosphorylated (p) Akt, ERK and JNK. E-cadherin and GAPDH work as normalization of membranous and cytosolic proteins respectively. The results shown are representative of 3 separate experiments

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Gut Pathogens

ISSN: 1757-4749