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Table 1 Primers and amplification conditions used for PCR

From: Oxidative stress due to Mycobacterium avium subspecies paratuberculosis (MAP) infection upregulates selenium-dependent GPx activity

Primer

Oligonucleotide sequence (5′–3′)

Gene

Amplification conditions

Product size (bp)

References

P90, P91

GTTCGGGGCCGTCGCTTAGG, GAGGTCGATCGCCCACGTGA

IS900

95 °C for 5 min, then 34 cycles of 95 °C for 1 min, 58 °C for 1.5 min, 72 °C for 1.5 min. Final extension of 10 min at 72 °C

398

Naser et al. [3]

AV1, AV2

ATGTGGTTGCTGTGTTGGATGG, CCGCCGCAATCAACTCCAG

IS900

95 °C for 5 min, then 34 cycles of 95 °C for 1 min, 58 °C for 1.5 min, 72 °C for 1.5 min. Final extension of 10 min at 72 °C

298

Naser et al. [3]