Table 1 Details of PCR-REA
PCR | Target | Forward primer | Reverse primer | Predicted product size (bp) | Enzyme | subspecies | Predicted band sizes (bp) |
|---|---|---|---|---|---|---|---|
1 | IS900 | P90 | P91 | 413 | AlwI | Map | 147,266 |
2 | IS1311 | M56 | M119 | 608 | HinfI/MseI | Map S | 285,323 |
| Â | Â | Â | Â | Â | Â | Map C | 67,218,285,323 |
| Â | Â | Â | Â | Â | Â | Map B | 67,218,323 |
| Â | Â | Â | Â | Â | Â | M. avium subsp. avium | 134,189,285 |