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Table 2 Size of PCR products at Sal20 locus

From: Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis

Isolate

No. of repeats

Predicted PCR product size (bp)

Sizing of PCR products by Agilent 2100 bioanalyzer

The low marker and upper marker, ladder included in DNA 1000 LabChip Kit

The new low marker and upper marker, 3 bp ladder

Size (bp)

Deviation (bp)

Size (bp)

Deviation (bp)

First

Second

Third

First

Second

Third

GZ73-1

9

172

182

181

183

9–11

172

172

172

0

GX97-202

13

184

194

193

195

9–11

184

184

184

0

GZ85-2

14

187

198

196

198

9–11

187

187

187

0

JS01-104

15

190

199

200

202

9–11

190

190

190

0

GZ82-16

16

193

203

202

202

9–11

193

193

193

0

GZ81-15

17

196

206

205

206

9–10

196

196

196

0

GZ84-1

18

199

209

208

210

9–11

199

199

199

0

GX98-213

19

202

212

211

213

9–11

202

202

202

0

ZJ00-21

20

205

216

215

217

10–12

205

205

205

0