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Fig. 3 | Gut Pathogens

Fig. 3

From: Examination of the effects of Campylobacter concisus zonula occludens toxin on intestinal epithelial cells and macrophages

Fig. 3

The effect of C. concisus Zot on the production of TNF-α and IL-8 in HT-29 and THP-1 macrophage-like cells. HT-29 cells (a and b) HT-29 cells were treated with EP and EP-ZotP14UCO-S1 proteins at 50 μg/100 μl for 24 h. The supernatants were collected and the levels of TNF-α and IL-8 were measured using commercially available ELISA kits. TNF-α and IL-8 levels in P1CDO3 treated cells were 34 ± 8 and 246 ± 27 pg/ml respectively. THP-1 macrophage-like cells (c and d) THP-1 macrophage-like cells were treated with EP and EP-ZotP14UCO-S1 proteins at 50 μg/100 μl for 2 h. The supernatants were collected and the levels of TNF-α and IL-8 were measured using commercially available ELISA kits. Data are shown as the mean ± SD from triplicates and are representative of two independent experiments. The value in the negative control (cells without any treatment) was subtracted from the data presented in this figure. TNF-α and IL-8 levels in P1CDO3 treated cells were 1738 ± 230 and 1708 ± 187 pg/ml respectively. *P < 0.05, **P < 0.01 and ***P < 0.001. EP-ZotP14UCO-S1: constituted of E. coli proteins and the Zot of C. concisus strain P14UCO-S1 expressed in E. coli. EP control E. coli proteins

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