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Fig. 1 | Gut Pathogens

Fig. 1

From: Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples

Fig. 1

Contaminating endogenous DNA from DNA extraction and processing kits produced CT values of 28–30 in no template controls using universal rRNA gene primers. Samples containing low bacterial biomass and high levels of competing human DNA, such as intestinal submucosal or peripheral blood samples, often produce CT values greater than the no template controls. Bacteria are discernable from background only in samples containing a high bacterial biomass such as intestinal mucosal tissues. Reagent contamination interferes and prevents bacterial quantitation based on the rRNA genes in low bacterial biomass samples

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Gut Pathogens

ISSN: 1757-4749