Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples

Table 1 Bacterial families and genera identified in peripheral blood DNA extraction kits processed with molecular biology grade water instead of blood

Family	Genus	Relative %^a
Actinomycetaceae	Actinomyces spp.	0.082576
Bacillaceae	Geobacillus spp.	56.39967
Bacteroidaceae	Bacteroides spp.	0.743187
Bifidobacteriaceae	Bifidobacterium spp.^b	0.082576
Carnobacteriaceae	Granulicatella spp.^b	0.165153
Comamonadaceae	Alicycliphilus spp.^b	0.082576
Comamonadaceae	Pelomonas spp.	3.220479
Coriobacteriaceae	Atopobium spp.^b	0.082576
Enterobacteriaceae	Escherichia spp.	0.247729
Enterococcaceae	Enterococcus spp.^b	0.165153
Erysipelotrichaceae	Erysipelatoclostridium spp.^b	0.082576
[Gemellaceae]	Gemella spp.^b	0.082576
Lachnospiraceae	Blautia spp.	1.156069
	Dorea spp.	0.247729
	Roseburia spp.	0.247729
Lactobacillaceae	Lactobacillus spp.^b	0.082576
Microbacteriaceae	Agrococcus spp.	0.247729
Oxalobacteraceae	Herbaspirillum spp.^b	0.082576
Pasteurellaceae	Haemophilus spp.	0.495458
Phyllobacteriaceae	Phyllobacterium spp.^b	0.082576
Porphyromonadaceae	Parabacteroides spp.^b	0.082576
Propionibacteriaceae	Propionibacterium spp.	34.10405
Pseudomonadaceae	Pseudomonas spp.^b	0.082576
Rhodobacteraceae	Ruegeria spp.^b	0.082576
Rikenellaceae	Alistipes spp.^b	0.082576
Streptococcaceae	Streptococcus spp.	1.156069
Veillonellaceae	Dialister spp.	0.082576
	Quinella spp.	0.082576
	Veillonella spp.	0.165153

Bacterial DNA belonging to 29 genera and 39 tentative species were found to contaminate peripheral blood DNA extraction kits
See Additional file 1: Table S1 for list of tentative species identification
^aRelative percent prevalence are average of multiple lots
^bDetection of organism was lot dependent and not present in all lots

ISSN: 1757-4749