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Table 2 Bacterial families and genera identified within the MoBio DNA extraction kit DNA from 81 bacterial genera and 108 tentative species were identified as inherent contaminants

From: Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples

Family Genera Relative %a
Acetobacteraceae Roseomonas 1.71
Actinomycetaceae Actinomyces 0.03
Aerococcaceae Abiotrophia 1.13
Alcanivoracaceae Alcanivorax b 0.005
Alicyclobacillaceae Tumebacillus 3.7
Alphaproteobacteria, unclassified Candidatus_alysiosphaera b 0.003
Aurantimonadaceae Aurantimonas b 0.002
Bacillaceae Bacillus 0.3
Bacillales family xi. incertae sedis Gemella b 0.005
Bacteroidaceae Bacteroides 0.08
Bifidobacteriaceae Bifidobacterium b 0.003
Bradyrhizobiaceae Afipia b 0.001
Bradyrhizobium 0.51
Burkholderiaceae Burkholderia 0.12
Roseateles 1.31
Cardiobacteriaceae Cardiobacterium 0.014
Carnobacteriaceae Granulicatella 0.02
Trichococcus b 0.002
Christensenellaceae Christensenella b 0.002
Clostridiaceae Clostridium 0.024
Clostridiales, unclassified Flavonifractor b 0.002
Pseudoflavonifractor b 0.002
Clostridiales family xi. incertae sedis Anaerococcus b 0.004
Comamonadaceae Comamonas 0.037
Coprococcus b 0.002
Curvibacter 0.155
Pseudorhodoferax 0.32
Coriobacteriaceae Atopobium b 0.002
Collinsella b 0.005
Corynebacteriaceae Corynebacterium 2.2
Eggerthellaceae Eggerthella b 0.007
Slackia b 0.002
Enterobacteriaceae Escherichia 0.03
Escherichia_Shigella b 0.003
Klebsiella 0.02
Serratia 0.007
Enterococcaceae Enterococcus 0.008
Erysipelotrichaceae Catenibacterium b 0.002
Erysipelatoclostridium 0.02
Solobacterium b 0.005
Turicibacter 0.021
Eubacteriaceae Eubacterium b 0.003
Flavobacteriaceae Capnocytophaga 0.21
Chryseobacterium 0.33
Cloacibacterium 0.003
Fusobacteriaceae Fusobacterium 0.28
Geodermatophilaceae Blastococcus 0.25
Gordoniaceae Gordonia 0.02
Halanaerobiaceae Halocella b 0.005
Lachnospiraceae Blautia 0.02
Johnsonella b 0.002
Lachnoanaerobaculum 0.23
Lachnoclostridium b 0.005
Lachnospira 0.007
Moryella b 0.002
Pseudobutyrivibrio 0.02
Roseburia 0.009
Tyzzerella b 0.002
Lactobacillaceae Lactobacillus 0.009
Leptotrichiaceae Leptotrichia 0.28
Methylobacteriaceae Methylobacterium 11.7
Methylocystaceae Methylopila b 0.003
Microbacteriaceae Pseudoclavibacter b 0.005
Micrococcaceae Arthrobacter 0.03
Rothia b 0.002
Micromonosporaceae Pilimelia b 0.002
Moraxellaceae Enhydrobacter 1.95
Neisseriaceae Kingella b 0.002
Neisseria 0.05
Oxalobacteraceae Janthinobacterium 0.002
Massilia 0.11
Oxalobacter 2.3
Paenibacillaceae Brevibacillus 0.03
Paenibacillus b 0.003
Pasteurellaceae Haemophilus 0.26
Pelagibacteraceae Candidatus pelagibacter b 0.002
Peptococcaceae Peptococcus b 0.003
Peptoniphilaceae Parvimonas 0.02
Peptoniphilus b 0.002
Peptostreptococcaceae Intestinibacter 0.043
Phyllobacteriaceae Phyllobacterium 0.3
Porphyromonadaceae Porphyromonas b 0.003
Prevotellaceae Prevotella 10.4
Xylanibacter b 0.002
Propionibacteriaceae Propionibacterium 15.8
Pseudomonadaceae Pseudomonas 0.009
Rhodobacteraceae Rubellimicrobium 0.78
Rikenellaceae Alistipes 0.05
Ruminococcaceae Anaerotruncus b 0.001
Faecalibacterium 0.02
Fastidiosipila b 0.003
Oscillospira b 0.002
Papillibacter b 0.003
Ruminiclostridium b 0.003
Ruminococcus 0.007
Sphingobacteriaceae Sphingobacterium b 0.003
Staphylococcaceae Staphylococcus 5.2
Streptococcaceae Streptococcus 13.4
Sutterellaceae Parasutterella b 0.005
Thermaceae Meiothermus 0.06
tm7 (candidate division) tm7 (candidate division) 0.44
Veillonellaceae Dialister b 0.003
Megasphaera 1.53
Selenomonas 0.44
Veillonella 20.4
Xanthomonadaceae Stenotrophomonas 1.25
  1. These taxa are unlikely to have been introduced by PCR kit reagents or contamination on the bench, and so are likely to have originated from the DNA extraction kit and during the DNA extraction process
  2. See Additional file 1: Table S1 for list of tentative species identification
  3. aRelative percent prevalence are average of multiple lots
  4. bDetection of organism was lot dependent and not present in all lots