Fig. 1
From: Calcium binding protects E-cadherin from cleavage by Helicobacter pylori HtrA
Calcium ions change the HtrA-mediated E-cadherin cleavage pattern. a Model of the domain structure of human E-cadherin (hCdh1). EC extracellular domain, TD transmembrane domain, IC intracellular domain, NTF soluble N-terminal fragment, open arrows signature sites for HtrA, black arrow cleavage site in the linker region. b 100 ng rCdh1 was incubated with 200 ng HpHtrA and, where indicated, with 125 µM EDTA or 100 µM CaCl2. Full-length E-cadherin (rCdh1FL, ~125 kDa) and rCdh1 fragments were detected by western blot using an antibody against the EC5 domain of E-cadherin. HpHtrA and the auto-processed short HpHtrA (HpHtrAs) were probed using a polyclonal antibody to show equal loading