Fig. 4
From: Calcium binding protects E-cadherin from cleavage by Helicobacter pylori HtrA
Calcium does not interfere with oligomerization of HpHtrA. a 3 µg HpHtrA were preincubated with increasing concentrations of calcium ions, as indicated, followed by separation by SDS-PAGE under non-reducing conditions. Proteins were Coomassie-stained to visualize HpHtrA in its monomeric and multimeric (multi) forms. b 200 ng HpHtrA were incubated with different concentrations of calcium ions for 16 h. Proteolytic activities of HpHtrA and HpHtrAs were analyzed by casein zymography