Fig. 2From: RHB-104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of Crohn’s disease associated with Mycobacterium paratuberculosis Nested PCR analysis of DNA from representative mycobacterial cultures. IS900-based nested PCR (nPCR) was performed on DNA extracts from MAP and other mycobacterial cultures. PCR products were analyzed on 2 % agarose gel and 298 bp amplified fragment is considered positive for MAP (lane 20). 1: UCF 5, 2: UCF 8, 3: MAP 7, 4: MAP 8B, 5: MAP Ben, 6: MAP Kay, 7: MAP Linda, 8: MAP Para, 9: MAP UCF 3, 10: MAP UCF 4, 11: MAP UCF 5, 12: MAP UCF 7, 13: MAP UCF 8, 14: M. avium JF 7, 15: M. avium JF 8, 16: MAP Para 18, 17: M. avium avium, 18: M. intracellulari LM1A, 19: M. smegmatis, 20: M. tuberculosis Back to article page