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Table 2 Susceptibility of mycobacterial strains to CLA, CLO, and RIF at their concentrations in the MIC of CLA–CLO–RIF (RHB-104 active ingredients)

From: RHB-104 triple antibiotics combination in culture is bactericidal and should be effective for treatment of Crohn’s disease associated with Mycobacterium paratuberculosis

Organism Source MIC Comparison analysis
CLA–CLO–RIF CLA (63.3 %) CLO (6.7 %) RIF (30 %)
μg/mL μg/mL Susceptibility μg/mL Susceptibility μg/mL Susceptibility
Clinical MAP strains
 MAP UCF 3 Tissue 0.25 0.158 R 0.017 R 0.075 R
 MAP UCF 4 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP UCF 5 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP UCF 7 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP UCF 8 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP Strain 1 Milk 0.25 0.633 R 0.067 R 0.3 R
 MAP Strain 3 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP Strain 7 Tissue 0.25 0.633 R 0.067 R 0.3 R
 MAP Strain 8B Blood 0.25 0.633 R 0.067 R 0.3 R
 MAP MS 137 Tissue 4 2.532 R 0.268 R 1.2 R
 MAP MS 185 Tissue 4 2.532 R 0.268 R 1.2 R
 MAP Para 18 ATCC 19698 0.25 0.633 R 0.067 R 0.3 R
 MAP Ben ATCC 43544 0.25 0.633 R 0.067 R 0.3 R
 MAP Kay ATCC C286 0.25 0.633 R 0.067 R 0.3 R
 MAP Linda ATCC 43015 0.25 0.633 R 0.067 R 0.3 R
Non-MAP strains
 M. avium ATCC 25291 4 2.532 R 0.268 R 1.2 R
 M. avium JF 5 Faeces 4 2.532 R 0.268 R 1.2 R
 M. avium JF 6 Faeces 4 2.532 R 0.268 R 1.2 R
 M. avium JF 8 Blood 4 2.532 R 0.268 R 1.2 R
 M. intracellulare LM1-A Sputum 4 3.798 R 0.402 R 1.8 R
 M-Smegmatis ATCC 27199 6 3.798 R 0.402 R 1.8 R
 M. chelonae subsp. chelonea   >10a 6.33 R 0.67 R 3 R
 M. fortuitum subsp. fortuitum   >10a 6.33 R 0.67 R 3 R
  1. MIC minimum inhibitory concentration, CLA clarithromycin, CLO clofazimine, RIF rifabutin, Suscep susceptibility
  2. aMIC was greater than 10 μg/mL and no higher drug concentrations were analyzed