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Table 1 List of primers and primer sequences used for detection of antimicrobial resistance genes

From: Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya

Antibiotic

Method

Primer

Sequence (5’–3’)

Amplicon length (bp)

Reference

Erythromycin

MAMA–PCRa

23SRNA-F

ERY2075-R

ERY2074-R

TTA GCT AAT GTT GCC CGT ACC G

TAG TAA AGG TCC ACG GGG TCG C

AGT AAA GGT CCA CGG GGT CTG G

485

[17]

PCR–RFLPa

F2-campy-23S

R2-campy-23S

AAT TGA TGG GGT TAG CAT TAG C

CAA CAA TGG CTC ATA TAC AAC TGG

316

[20]

Ciprofloxacin

MAMA–PCRb

CampyMAMAgyrA1

CampyMAMAgyrA5

TTT TTA GCA AAG ATT CTG AT

CAA AGC ATC ATA AAC TGC AA

265

[19]

MAMA–PCRc

GZgyrACcoli3F

CanpyMAMAgyrA8

TAT GAG CGA TAT TAT CGG TC

TAA GGC ATC GTA AAC AGC CA

192

[24]

Tetracycline

tet(O) PCR

DMT 1

DMT 2

GGC GTT TTG TTT ATG TGC G

ATG GAC AAC CCG ACA GAA GC

559

[25]

tet(A) PCR

Tet(A)-F

Tet(A)-R

GTG AAA CCC AAC ATA CCC C

GAA GGC AAG CAG GAT GTA G

888

[18]

tet(A) PCR

tet-A-1

tet-A-2

GCT CAC GTT GAC GCA GGA AAG

ATC GTC ATT GTC CGT TAC

486

This study

  1. a23S rRNA gene mutation
  2. b gyrA gene mutation Campylobacter jejuni
  3. c gyrA gene mutation Campylobacter coli