Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya

Table 1 List of primers and primer sequences used for detection of antimicrobial resistance genes

Antibiotic	Method	Primer	Sequence (5’–3’)	Amplicon length (bp)	Reference
Erythromycin	MAMA–PCR^a	23SRNA-F ERY2075-R ERY2074-R	TTA GCT AAT GTT GCC CGT ACC G TAG TAA AGG TCC ACG GGG TCG C AGT AAA GGT CCA CGG GGT CTG G	485	[17]
Erythromycin	PCR–RFLP^a	F2-campy-23S R2-campy-23S	AAT TGA TGG GGT TAG CAT TAG C CAA CAA TGG CTC ATA TAC AAC TGG	316	[20]
Ciprofloxacin	MAMA–PCR^b	CampyMAMAgyrA1 CampyMAMAgyrA5	TTT TTA GCA AAG ATT CTG AT CAA AGC ATC ATA AAC TGC AA	265	[19]
Ciprofloxacin	MAMA–PCR^c	GZgyrACcoli3F CanpyMAMAgyrA8	TAT GAG CGA TAT TAT CGG TC TAA GGC ATC GTA AAC AGC CA	192	[24]
Tetracycline	tet(O) PCR	DMT 1 DMT 2	GGC GTT TTG TTT ATG TGC G ATG GAC AAC CCG ACA GAA GC	559	[25]
	tet(A) PCR	Tet(A)-F Tet(A)-R	GTG AAA CCC AAC ATA CCC C GAA GGC AAG CAG GAT GTA G	888	[18]
	tet(A) PCR	tet-A-1 tet-A-2	GCT CAC GTT GAC GCA GGA AAG ATC GTC ATT GTC CGT TAC	486	This study

ISSN: 1757-4749