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Table 1 List of primers and primer sequences used for detection of antimicrobial resistance genes

From: Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya

Antibiotic Method Primer Sequence (5’–3’) Amplicon length (bp) Reference
Erythromycin MAMA–PCRa 23SRNA-F
ERY2075-R
ERY2074-R
TTA GCT AAT GTT GCC CGT ACC G
TAG TAA AGG TCC ACG GGG TCG C
AGT AAA GGT CCA CGG GGT CTG G
485 [17]
PCR–RFLPa F2-campy-23S
R2-campy-23S
AAT TGA TGG GGT TAG CAT TAG C
CAA CAA TGG CTC ATA TAC AAC TGG
316 [20]
Ciprofloxacin MAMA–PCRb CampyMAMAgyrA1
CampyMAMAgyrA5
TTT TTA GCA AAG ATT CTG AT
CAA AGC ATC ATA AAC TGC AA
265 [19]
MAMA–PCRc GZgyrACcoli3F
CanpyMAMAgyrA8
TAT GAG CGA TAT TAT CGG TC
TAA GGC ATC GTA AAC AGC CA
192 [24]
Tetracycline tet(O) PCR DMT 1
DMT 2
GGC GTT TTG TTT ATG TGC G
ATG GAC AAC CCG ACA GAA GC
559 [25]
tet(A) PCR Tet(A)-F
Tet(A)-R
GTG AAA CCC AAC ATA CCC C
GAA GGC AAG CAG GAT GTA G
888 [18]
tet(A) PCR tet-A-1
tet-A-2
GCT CAC GTT GAC GCA GGA AAG
ATC GTC ATT GTC CGT TAC
486 This study
  1. a23S rRNA gene mutation
  2. b gyrA gene mutation Campylobacter jejuni
  3. c gyrA gene mutation Campylobacter coli