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Table 1 Clinical signs and rotavirus fecal shedding in Gn pigs after VirHRV challenge

From: Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs

Group N Clinical signs Fecal virus shedding (by CCIF and/or ELISA)
% with diarrheaa,* Mean days to onsetb,** Mean duration dayse,** Mean cumulative scorec,** % shedding virus* Mean days to onsetb,** Mean duration dayse,** Mean peak titer (FFU/ml)
HHGM 7 57.1 4.3 (0.23)d 1.3 (0.57)d 7.7 (0.77)d 42.85* 1.7 (0.2)d 1.6 (0.7)d 657.4
UHGM 6 83.3 2.3 (1.1) 2.0 (0.89) 11.2 (1.2) 100* 2.4 (0.3) 3.0 (0.63) 1683.7
  1. ELISA enzyme-linked immunosorbent assay, CCIF cell culture immunofluorescent assay, FFU fluorescence forming unit
  2. * Fisher’s exact test was used for comparisons. Asterisk indicate significant differences among groups (n = 6–7; p < 0.05)
  3. ** Kruskal–Wallis rank sum test was used for comparisons. No statistically significant differences were observed between the groups
  4. a Pigs with daily fecal scores of ≥2 were considered diarrheic. Fecal consistency was scored as follows: 0, normal; 1, pasty; 2, semiliquid; and 3, liquid
  5. b In the groups where some but not all pigs had diarrhea or shedding, the onset of diarrhea or shedding for non-diarrheic/shedding pigs was designated as 8 for calculating the mean days to onset
  6. c Mean cumulative score calculation included all the pigs in each groups
  7. d Standard error of the mean
  8. e For days of diarrhea and virus shedding, if no diarrhea or virus shedding until the euthanasia day (PCD7), the duration days were recorded as 0