Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs

Table 1 Clinical signs and rotavirus fecal shedding in Gn pigs after VirHRV challenge

Group	N	Clinical signs				Fecal virus shedding (by CCIF and/or ELISA)
Group	N	% with diarrhea^a,*	Mean days to onset^b,**	Mean duration days^e,**	Mean cumulative score^c,**	% shedding virus*	Mean days to onset^b,**	Mean duration days^e,**	Mean peak titer (FFU/ml)
HHGM	7	57.1	4.3 (0.23)^d	1.3 (0.57)^d	7.7 (0.77)^d	42.85*	1.7 (0.2)^d	1.6 (0.7)^d	657.4
UHGM	6	83.3	2.3 (1.1)	2.0 (0.89)	11.2 (1.2)	100*	2.4 (0.3)	3.0 (0.63)	1683.7

ELISA enzyme-linked immunosorbent assay, CCIF cell culture immunofluorescent assay, FFU fluorescence forming unit
* Fisher’s exact test was used for comparisons. Asterisk indicate significant differences among groups (n = 6–7; p < 0.05)
** Kruskal–Wallis rank sum test was used for comparisons. No statistically significant differences were observed between the groups
^a Pigs with daily fecal scores of ≥2 were considered diarrheic. Fecal consistency was scored as follows: 0, normal; 1, pasty; 2, semiliquid; and 3, liquid
^b In the groups where some but not all pigs had diarrhea or shedding, the onset of diarrhea or shedding for non-diarrheic/shedding pigs was designated as 8 for calculating the mean days to onset
^c Mean cumulative score calculation included all the pigs in each groups
^d Standard error of the mean
^e For days of diarrhea and virus shedding, if no diarrhea or virus shedding until the euthanasia day (PCD7), the duration days were recorded as 0

ISSN: 1757-4749