Table 1 Primers used for DNA sequencing of H. pylori cagA and vacA in this study
Gene | Primer | Sequence (5′–3′) | References |
|---|---|---|---|
cagA | Luni 1 | ACATTTTGGCTAAATAAACACTG | [39] |
R5280 | GTTGCACGCATTTTCCCTTGATC | [39] | |
L2(+) | AAGGAGAAACAATGACTAACGAAACTATTG | [40] | |
L2(−) | TCCTTTAAGATTTTTGGAAACCACCTTTTG | [40] | |
hp552−R2 | CTCTTACTCACCCTTGTATCCTCGCATA | In this study | |
hp552−R1 | GCCTGGATCGCTCAAACTTGGCATGC | In this study | |
cagA−R2 | GGGTGTTGATTTTAGACGGATC | In this study | |
cagA−F9 | TCGTTCAAGTTTTCCACCAAGTTGA | In this study | |
cagA−F11 | CAATCAAGAGGCTAGTAAGGAAG | In this study | |
cagA−F6 | CTCTCAAAGATTATGGGAAAAAA | In this study | |
cagA−F10 | ACAATAACGTTCTATCTTCTGTGCT | In this study | |
cagA−F8 | AAAGATCCGTCTAAAATCAACACCC | In this study | |
TF | ACCCTAGTCGGTAATG | [20] | |
TR | TATCAGAAGCTAAAAC | [20] | |
OMF | AGCAAAAAGCGACCTTGAAA | [20] | |
OMR | ATTCACGAGCTTGAGCCACT | [20] | |
vacA | vacAcysS−F2 | GAATTTCAATGAAGAAGACTTGTT | In this study |
vacA−R4 | GCGGTGTGTTTGTTGTATTTCCAT | In this study | |
VA1−F | ATGGAAATACAACAAACACAC | [22] | |
VAG−R | GCGTCAAAATAATTCCAAGGC | [25] | |
VAG−F | CAATCTTGTCCAATCAAGCGAG | [25] | |
VA3−F | GGTCAAAATGCGGTCATGG | [22] | |
vacAfecE−R2 | CTTATTGTTACTGGATGAGCCTA | In this study |