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Table 1 Gene copy (GC) number of CD strain 1296 in proximal (PC) and transverse-distal (TDC) colon reactors of model 1

From: Clostridium difficile colonization and antibiotics response in PolyFermS continuous model mimicking elderly intestinal fermentation

Days post CD instillation

Reactors

PC (GC mL−1)

TDC (GC mL−1)

0.25

7.4

7.3

1

5.7

6.2

2

4.4

5.1

3

ND

ND

4

ND

ND

5

ND

ND

6

ND

ND

7

ND

ND

8

ND

ND

9

ND

6.0

10

ND

6.0

11

ND

6.4

12

ND

6.9

13

ND

7.1

14

ND

7.5

15

ND

7.8

16

ND

7.8

  1. Data are mean log10 16S rRNA GC mL−1 fermentation effluent from 6 h after CD vegetative cells inoculation (log10 9.8 cfu in 250 mL volume PC reactor) and during the following 16 days. Samples were analyzed in duplicate. The detection limit of qPCR of CD was log10 4.4 GC mL−1
  2. PC, proximal colon; TDC, transverse-distal colon; CD, C. difficile; ND, not detected
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