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Table 2 Gene copy (GC) number of bacterial groups in TDC reactors of model 2 during antibiotic treatment [A, B and E] and recovery [F] periods

From: Clostridium difficile colonization and antibiotics response in PolyFermS continuous model mimicking elderly intestinal fermentation

 

Total 16S rRNA gene

Bacteroides spp.

Entero-bacteriaceae

Lactobacillus spp.

Bifidobacterium spp.

F. prausnitzii

Clostridium cluster IV

Roseburia spp./E. rectale

Period A (CRO I)

 CR

11.3 ± 0.2

10.3 ± 0.1

9.8 ± 0.2

7.3 ± 0.3

8.4 ± 0.3

9.0 ± 0.1

9.8 ± 0.2

7.4 ± 0.1

 TR1

11.2 ± 0.2

10.1 ± 0.1

9.7 ± 0.1

7.3 ± 0.2

8.3 ± 0.4

8.9 ± 0.1

9.7 ± 0.2

7.4 ± 0.1

 TR3

11.4 ± 0.07

10.1 ± 0.1

9.8 ± 0.3

7.5 ± 0.1

8.2 ± 0.1

8.9 ± 0.2

9.5 ± 0.5

7.4 ± 0.1

Period B (CRO II)

 CR

11.3 ± 0.4

10.3 ± 0.5

9.6 ± 0.3

8.3 ± 0.3

8.2 ± 0.2

8.9 ± 0.3

9.1 ± 0.2

6.5 ± 0.1

 TR1

11.3 ± 0.04

10.6 ± 0.3

9.7 ± 0.2

8.3 ± 0.1

6.7 ± 0.2*

8.6 ± 0.4

8.8 ± 0.1*

6.0 ± 0.4

 TR3

11.4 ± 0.1

10.5 ± 0.1

9.5 ± 0.1

8.5 ± 0.2

6.7 ± 0.3*

8.6 ± 0.5

8.8 ± 0.1*

6.1 ± 0.2*

Period E (MTZ)

 CR

10.9 ± 0.2

9.6 ± 0.2

10.4 ± 0.2

7.6 ± 0.2

7.1 ± 0.3

9.1 ± 0.1

9.7 ± 0.1

5.8 ± 0.1

 TR1

11.2 ± 0.2

9.5 ± 0.2

9.4 ± 0.5*

7.7 ± 0.3

9.5 ± 0.2*

5.7 ± 0.1*

5.9 ± 0.2*

5.1 ± 0.2*

 TR4

11.1 ± 0.1

9.7 ± 0.2

9.3 ± 0.3*

8.1 ± 0.3

9.4 ± 0.3*

6.7 ± 0.1*

6.7 ± 0.2*

5.0 ± 0.2*

Period F (REC)

 CR

11.3 ± 0.2

10.2 ± 0.1

10.1 ± 0.4

7.2 ± 0.5

7.1 ± 0.2

8.3 ± 0.2

9.7 ± 0.2

6.4 ± 0.1

 TR1

11.7 ± 0.3

10.3 ± 0.1

10.1 ± 0.3

7.4 ± 0.4

7.6 ± 0.3*

8.2 ± 0.3

8.5 ± 0.03*

6.3 ± 0.1

 TR4

11.3 ± 0.1

10.1 ± 0.2

10.3 ± 0.4

7.4 ± 0.1

7.1 ± 0.1

7.2 ± 0.2*

8.6 ± 0.2*

5.5 ± 0.02*

  1. Data are mean log10 GC mL−1 fermentation effluent ± SD of the last 3 days of the treatment periods; samples were analyzed in duplicates
  2. Means with an asterisk differ significantly from the control reactor within the same bacterial group (p < 0.05)
  3. CR control reactor, TR test reactor, CRO ceftriaxone, MTZ metronidazole, REC recovery