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Table 3 Metabolites concentration (mM) and ratios (%) measured by HPLC in model 2 during antibiotic treatment [A, B and E] and recovery [F] periods

From: Clostridium difficile colonization and antibiotics response in PolyFermS continuous model mimicking elderly intestinal fermentation

  Acetate Propionate Butyrate Formate Total metabolites SCFA Ratios (%)
Acetate Propionate Butyrate
Period A (CRO I)
 CR 75.7 ± 4.8 33.4 ± 2.5 38.4 ± 2.2 ND 147.5 ± 5.8 51.3 22.6 26.0
 TR1 75.0 ± 3.0 36.7 ± 4.0 37.4 ± 0.9 ND 148.4 ± 6.4 20.5 24.7 24.7
 TR3 76.5 ± 4.1 33.5 ± 2.0 35.8 ± 1.3 ND 145.8 ± 4.7 52.5 23.0 24.6
Period B (CRO II)
 CR 91.2 ± 0.9 43.6 ± 2.2 18.4 ± 4.6 ND 153.2 ± 5.2 59.5 28.5 12.0
 TR1 93.5 ± 7.4 47.2 ± 4.3 19.5 ± 2.6 ND 160.2 ± 8.9 58.4 29.5 12.2
 TR3 97.0 ± 5.1* 45.6 ± 4.3 19.6 ± 2.1 ND 162.2 ± 7.0 97 45.6 19.6
Period E (MTZ)
 CR 99.2 ± 2.4 37.0 ± 1.2 13.3 ± 0.5 ND 149.5 ± 2.7 66.4 24.7 8.9
 TR1 44.1 ± 0.7* 35.9 ± 1.8 ND 10.6 ± 1.0 90.6 ± 2.2* 55.1 44.9 0
 TR4 45.6 ± 2.5* 37.0 ± 1.3 ND 11.3 ± 1.6 93.9 ± 3.2* 55.2 44.8 0
Period F (REC)
 CR 92.6 ± 10.9 39.9 ± 6.2 13.4 ± 0.2 ND 145.9 ± 12.5 63.5 27.3 9.2
 TR1 83.7 ± 0.4 43.8 ± 2.4 13.4 ± 0.7 ND 140.9 ± 2.5 59.4 31.1 9.5
 TR4 88.4 ± 8.6 44.8 ± 9.8 13.5 ± 1.6 ND 146.7 ± 13.1 60.3 30.5 9.2
  1. Data are mean ± SD of the three last days of treatment periods; samples were analyzed in duplicates
  2. Means with an asterisk (*) differ significantly from the control reactor within the same metabolite (p < 0.05)
  3. CR control reactor, TR test reactor, SCFA short-chain fatty acids, CRO ceftriaxone, MTZ metronidazole, REC recovery, ND not detected