Epidemiological, molecular characterization and antibiotic resistance of Salmonella enterica serovars isolated from chicken farms in Egypt

Table 4 Primer sequences and their corresponding genes used for detection of antimicrobial resistant genes for S. enterica serovars

Gene	Primer	Nucleotide sequence (5–3)	Annealing (°C)	Amplicon size (bp)	Reference
aadA1	F	TATCAGAGGTAGTTGGCGTCAT	54	484	[62]
aadA1	R	GTTCCATAGCGTTAAGGTTTCATT	54	484	[62]
aadA2	F	TGTTGGTTACTGTGGCCGTA	62	622	[62]
aadA2	R	GATCTCGCCTTTCACAAAGC	62	622	[62]
aadB	F	GAGCGAAATCTGCCGCTCTGG	61	319	[62]
aadB	R	CTGTTACAACGGACTGGCCGC	61	319	[62]
aacC	F	GGCGCGATCAACGAATTTATCCGA	58	488	[28]
aacC	R	CCATTCGATGCCGAAGGAAACGAT	58	488	[28]
blaTEM	F	CATTTCCGTGTCGCCCTTAT	55	793	[62]
blaTEM	R	TCCATAGTTGCCTGACTCCC	55	793	[62]
cat1	F	CTT GTC GCC TTG CGT ATA AT	53	508	[27]
cat1	R	ATC CCA ATG GCA TCG TAA AG	53	508	[27]
cat2	F	CCGGATTGACCTGAATACCT	56	572	[62]
cat2	R	TCACATACTGCATGATGAAC	56	572	[62]
dfrI	F	GTGAAACTATCACTAATGGTAGCT	54	470	[62]
dfrI	R	ACCCTTTTGCCAGATTTGGTAACT	54	470	[62]
floR	F	AACCCGCCCTCTGGATCAAGTCAA	60	548	[62]
floR	R	CAAATCACGGGCCACGCTGTATC	60	548	[62]
strA	F	AGCAGAGCGCGCCTTCGCTC	59	684	[62]
strA	R	CCAAAGCCCACTTCACCGAC	59	684	[62]
strB	F	ATCGTCAAGGGATTGAAACC	49	509	[63]
strB	R	GGATCGTAGAACATATTGGC	49	509	[63]
sul1	F	TCACCGAGGACTCCTTCTTC	60	316	[62]
sul1	R	AATATCGGGATAGAGCGCAG	60	316	[62]
sul2	F	CGGTCCGGCATCCAGCAATCC	64	441	[62]
sul2	R	CGAGAGCCACGACCGCGCC	64	441	[62]
sul3	F	GAGCAAGATTTTTGGAATCG	51	799	[63]
sul3	R	CATCTGCAGCTAACCTAGGGCTTGGA	51	799	[63]
tetA	F	GCTACATCCTGCTTGCCTTC	55	210	[64]
tetA	R	CATAGATCGCCGTGAAGAGG	55	210	[64]
tetB	F	TTGGTTAGGGGCAAGTTTTG	53	659	[64]
tetB	R	GTAATGGGCCAATAACACCG	53	659	[64]
tetC	F	CTTGAGAGCCTTCAACCCAG	56	418	[64]
tetC	R	ATGGTCGTCATCTACCTGCC	56	418	[64]
intI	F	5′GGCATCCAAGCAGCAAGC-3′	55	2000	[65]
intI	R	AAGCAGACTTGACCTGAT	55	2000	[65]

ISSN: 1757-4749