Fig. 7
Cell death, adhesion, and migration of neutrophils against C. albicans. a Cell death assay. 105 C. albicans cells were suspended in RPMI and mixed with 5 × 105 neutrophils. C. albicans was counted every 30 min. Results are expressed as percent death. b Adhesion of neutrophils to C. albicans. C. albicans yeasts were incubated for 1 h with neutrophils. Results are expressed as percent neutrophils adhering to yeasts. *P < 0.05 for TLR1−/−, and TLR2−/− neutrophils versus wild-type (WT) neutrophils. c Migration of neutrophils towards C. albicans. The migration of neutrophils towards C. albicans was performed using a transwell assay. Results are expressed as the percent neutrophils migrating from the upper to the lower chamber. Data are the mean ± SE of three independent experiments (P < 0.05). *P < 0.05 for TLR1−/−, and TLR2−/− neutrophils versus wild-type neutrophils