Fig. 1
From: Differential regulation of riboflavin supply genes in Vibrio cholerae
Assessment of the transcriptional organization of rib genes in V. cholerae. PCR reactions were carried on cDNA from V. cholerae with primers designed to amplify the indicated gene junctions of rib genes and their flanking genes in the loci encoding ribD, ribE, ribA-COG3236 and ribH (a), ribA2 (b), ribB (c) and ribN (d). Each reaction was independently performed three times with the same results. + Template cDNA derived from RT-PCR with reverse transcriptase, − template cDNA derived from RT-PCR without reverse transcriptase (negative control), C PCR with chromosomal DNA as template (positive control), M molecular weight marker (basepairs). Isosceles triangle over the regulatory region indicates FMN riboswitch. Half Isosceles triangle indicates truncated FMN riboswitch