Fig. 4

Level of cytokines in mouse gastric tissues and supernatants from cultured splenocytes. Tissue homogenates of gastric tissue of different group of mice infected by different combination of H. pylori were subjected for analysis of IFN-γ, IL-12 and IL-17 production by ELISA (a). Results were expressed as pg/mg total protein. Splenocytes isolated from control and all H. pylori infected groups were re-stimulated with or without H. pylori WCP (2.5 mg/ml). Supernatants were collected 48 h after stimulation, and secreted cytokines were measured by ELISA (b). Results were expressed as pg/ml protein. Gastric tissues extract from controls and H. pylori infected mice were analyzed for immunosuppressive cytokines, IL-10 and TGF-β production by ELISA (c). IL-10 and TGF-β concentration (pg/mg) were expressed as pg/mg total protein Error bars ±SEM ***P < 0.001; **P < 0.01; *P < 0.05; NS, P = not significant versus control; n = 4