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Fig. 3 | Gut Pathogens

Fig. 3

From: Capsular polysaccharide inhibits adhesion of Bifidobacterium longum 105-A to enterocyte-like Caco-2 cells and phagocytosis by macrophages

Fig. 3

qPCR to confirm the deletion of cpsD and its influence on downstream genes. Relative expression of wild-type B. longum 105-A (white) against B. longum 105-A ∆cpsD (black) of BL105A_0405 (cpsD), BL105A_0406, BL105A_0408, BL105A_0414, and BL105A_0424 (as indicated numbers) were analyzed by qPCR using the 2 −∆∆CT method. The rnaP gene was used as housekeeping control to normalize the data. In comparison to the wild-type B. longum 105-A, the expression of BL105A_0405 (cpsD) gene was undetectable in the ΔcpsD mutant. The downstream gene BL105A_0406, was significantly decreased by approximately 0.46% (P < 0.001) in the ΔcpsD mutant. The expression of genes, BL105A_0408, BL105A_0414, and BL105A_0424, were also decreased to 20% (P < 0.01). Temporal operon name also indicated below. ND not detected

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