Skip to main content

Table 1 Infective dose administered, bacteriological and clinical findings in individual animals

From: Comparison of clinical and immunological findings in gnotobiotic piglets infected with Escherichia coli O104:H4 outbreak strain and EHEC O157:H7

Group animal number Infective dose (CFUs) CFUs/g stool day 4 p.i. CFUs/g stool day 5–6 p.i. OD Stx ELISA day 5–6 p.i. CFUs/g stool day 9–12 p.i. OD Stx ELISA day 9–12 p.i. Average clinical score (animal/group) Clinical signs, assessed at least once Day of death
Negative
 1 Mock Sterile Sterile n.a. Sterile 0.009 (N) 0.00 None 10
 2 Mock Sterile Sterile 0.025 (N) Sterile 0.012 (N) 0.11 Reduced appetite 9
 3 Mock Sterile Sterile 0.020 (N)    0.00 None 6a
        Ø 0.04   
E. coli O104:H4
 4 1.00 × 109 1.22 × 1010 2.81 × 1010 n.a. 8.60 × 108 4.538 (P) 0.53 Reduced appetite, dehydration 10
 5 1.00 × 109 8.17 × 109 6.88 × 1010 n.a. 2.62 × 109 3.993 (P) 0.51 Reduced appetite, dehydration, mild central nerval signs 10
 6 2.19 × 108 2.13 × 1010 3.38 × 107 n.a. Sterile 2.746 (P) 0.46 Reduced appetite, dehydration 10
 7 2.19 × 108 1.18 × 107 2.10 × 109 n.a. Sterile 3.492 (P) 0.52 Reduced appetite, mild central nerval signs 9
 8 2.19 × 108 3.13 × 109 1.63 × 109 4.122 (P)    0.07 Reduced appetite 6a
 9 2.19 × 108 5.58 × 109 1.11 × 1010 2.833 (P) 4.65 × 107 4.561 (P) 0.11 Reduced appetite, dehydration 12
        Ø 0.37   
E. coli O157:H7
 10 2.20 × 108 5.43 × 109   .    0.74 Severe neurological signs 4b
 11 2.20 × 108 1.02 × 1010 8.35 × 109 n.a. 5.95 × 109 4.599 (P) 0.67 Mild central nerval signs, dehydration 11
 12 2.08 × 108 1.95 × 107 2.50 × 107 4.358 (P)    0.30 Severe neurological signs 5b
 13 2.08 × 108 1.30 × 109 1.37 × 108 4.527 (P)    0.50 Dehydration 5b
        Ø 0.55   
  1. Interpretation Shiga toxin ELISA: optical density (OD) < 0.070 = negative; OD > 0.070/<0.100 = indefinite, ODs > 0.100 = positive
  2. n.a. not assessed, N negative, P positive
  3. aEuthanized early due to organisational reasons
  4. bEuthanized early due to severe clinical symptoms