Analysis of proteomes released from in vitro cultured eight Clostridium difficile PCR ribotypes revealed specific expression in PCR ribotypes 027 and 176 confirming their genetic relatedness and clinical importance at the proteomic level

Table 1 Characterisation of C. difficile isolates in the study

Nr.	Isolate number^a	Year of isolation	Patient age^b	Sex	RT	ST	Clade	Presence of toxin genes	tcdC truncation
1	2063	2015	82	M	001	3	1	tcdA, tcdB	No
2	2023	2015	73	M	005	6	1	tcdA, tcdB	No
3	1107	2014	25	F	010	15	1	Non-toxigenic	NA
4	2006	2015	74	M	012	54	1	tcdA, tcdB	No
5	1120	2014	33	F	014	2	1	tcdA, tcdB	No
6	854	2014	35	M	027	1	2	tcdA, tcdB, cdtA, cdtB	Δ117
7	2004	2015	54	M	078	11	5	tcdA, tcdB, cdtA, cdtB	C184T
8	2062	2015	84	F	176	1	2	tcdA, tcdB, cdtA, cdtB	Δ117

^aNumber in the Czech national C. difficile strain collection
^bAge of the patient at the time of isolation, RT-PCR-ribotype, ST-sequence type, tcdC truncation—single nucleotide polymorphism (SNP) resulting in TcdC protein truncation

ISSN: 1757-4749