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Table 1 Antimicrobial substances and their dilutions used to determine MICs

From: Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens

Antimicrobial substance Group of antimicrobial agent Diluent to prepare stock solution Range of concentration (µg/ml)
Ampicilin β-Lactams BR IIa 0.125–64
Tetracycline Tetracyclines 0.1 M HCl 1–512
Doxycycline 0.5–256
Erythromycin Macrolides Methanol 99.9% 0.125–64
Tylosin H2O 0.125–64
Lincomycin Lincosamides 2–1024
Streptomycin Aminoglicosides H2O 2–1024
Gentamicin BR IIa 1–512
Neomycin BR IIa 1–512
Chloramphenicol Chloramphenicol Methanol 50% 0.5–256
Tiamulin Semisynthetic derivative of pleuromutilin 0.5–256
Enrofloxacin Fluoroquinolones 0.5–512
Flumequine 0.1 M NH4OH 2–1024
  1. aBR II buffer: 16.73 g K2HPO4 and 0.523 g KH2PO4 diluted in 1000 ml of distilled H2O, pH 7.9