Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens

Table 1 Antimicrobial substances and their dilutions used to determine MICs

Antimicrobial substance	Group of antimicrobial agent	Diluent to prepare stock solution	Range of concentration (µg/ml)
Ampicilin	β-Lactams	BR II^a	0.125–64
Tetracycline	Tetracyclines	0.1 M HCl	1–512
Doxycycline	Tetracyclines	0.1 M HCl	0.5–256
Erythromycin	Macrolides	Methanol 99.9%	0.125–64
Tylosin	Macrolides	H₂O	0.125–64
Lincomycin	Lincosamides	H₂O	2–1024
Streptomycin	Aminoglicosides	H₂O	2–1024
Gentamicin		BR II^a	1–512
Neomycin		BR II^a	1–512
Chloramphenicol	Chloramphenicol	Methanol 50%	0.5–256
Tiamulin	Semisynthetic derivative of pleuromutilin	–	0.5–256
Enrofloxacin	Fluoroquinolones	–	0.5–512
Flumequine	Fluoroquinolones	0.1 M NH₄OH	2–1024

^aBR II buffer: 16.73 g K₂HPO₄ and 0.523 g KH₂PO₄ diluted in 1000 ml of distilled H₂O, pH 7.9

ISSN: 1757-4749