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Fig. 6 | Gut Pathogens

Fig. 6

From: Toll-like receptor-4 differentially mediates intestinal and extra-intestinal immune responses upon multi-drug resistant Pseudomonas aeruginosa association of IL10−/− mice with chronic colitis

Fig. 6

Colonic immune cell responses in IL10−/− mice lacking TLR4 following peroral association with multi-drug resistant P. aeruginosa. Conventionally colonized IL10 deficient (WT IL10−/−; closed circles) and TLR4 deficient IL10−/− mice (TLR4−/− × IL10−/−; closed triangles) were perorally challenged with a clinical multi-drug resistant P. aeruginosa (PA) strain at day (d) 0. Fourteen days thereafter, the average numbers of colonic a macrophages and monocytes (positive for F4/80), b T lymphocytes (positive for CD3), c regulatory T cells (positive for FOXP3), and d B lymphocytes (positive for B220) were determined from six high power fields (HPF, 400× magnification) per animal in immunohistochemically stained large intestinal paraffin sections. Naive (N) mice served as negative controls (open symbols). Numbers of mice (in parentheses), medians (black bars) and significance levels (p values) determined by the Mann–Whitney U test are indicated. Data shown were pooled from three independent experiments

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Gut Pathogens

ISSN: 1757-4749