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Fig. 7 | Gut Pathogens

Fig. 7

From: Toll-like receptor-4 differentially mediates intestinal and extra-intestinal immune responses upon multi-drug resistant Pseudomonas aeruginosa association of IL10−/− mice with chronic colitis

Fig. 7

Intestinal and splenic cytokine responses in IL10−/− mice lacking TLR4 following peroral association with multi-drug resistant P. aeruginosa. Conventionally colonized IL10 deficient (WT IL10−/−; closed circles) and TLR4 deficient IL10−/− mice (TLR4−/− × IL10−/−; closed triangles) were perorally challenged with a clinical multi-drug resistant P. aeruginosa (PA) strain at day (d) 0. Fourteen days thereafter, secretion of distinct pro-inflammatory mediators were measured in supernatants of ex vivo biopsies derived from colon (a nitric oxide (NO); d IFN-γ), mesenteric lymph nodes (MLN; b IFN-γ; e TNF) and spleen (c IL-12p70; e TNF). Naive (N) mice served as negative controls (open symbols). Numbers of mice (in parentheses), medians (black bars) and significance levels (p values) determined by the Mann–Whitney U test are indicated. Data shown were pooled from three independent experiments

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