Table 1 Primers used for detection of virulence genes and CRISPR locus of H. pylori
From: CRISPR-like sequences in Helicobacter pylori and application in genotyping
DNA region(s) amplified | Primer name | Primer sequence | Amplicon sizes(s) (bp) | References or sources |
|---|---|---|---|---|
vacA s1/s2 | VAI-F VAI-R | 5′-ATGGAAATACAACAAACACAC-3′ 5′-CTGCTTGAATGCGCCAAAC-3′ | 259/286 | [24] |
vacA m1/m2 | VAG-F VAG-R | 5′-CAATCTGTCCAATCAAGCGAG-3′ 5′-GCGTCAAAATAATTCCAAGG-3′ | 567/642 | [24] |
cagA | Cag5c-F Cag5c-R | 5′-GTTGATAACGCTGTCGCTTC-3′ 5′-GGGTTGTATGATATTTTCCATAA-3′ | 350 | [24] |
vapD | D1-F D2-R | 5′-AGAGATGCGGTGAATGG-3′ 5′-AAGCGTTATGAGTGGTGTG-3′ | 498 | [25] |
CRISPR locus | CRISPR-HP-F CRISPR-HP-R | 5′-ATGGGGGCTTTAGTTTCAG-3′ 5′-TAGCAAAAGGCGAACTTGA-3′ | Variable | This study |