Target gene | Primer | Sequence (5′–3′) | Annealing | Size (bp) |
---|---|---|---|---|
ipaH | ipaH-F | TGGAAAAACTCAGTGCCTCT | 55 °C [12] | 423 |
ipaH-R | CCAGTCCGTAAATTCATTCT | |||
ial | ial-F | GCTATAGCAGTGACATGG | 55 °C [13] | 320 |
ial-R | ACGAGTTCGAAGCACTC | |||
virB | virB-F | CGATAGATGGCGAGAAATTATATCCCG | 56 °C [14] | 766 |
virB-R | CGATCAAGAATCCCTAACAGAAGAATCAC | |||
virF | virF-F | AGCTCAGGCAATGAAACTTTGAC | 60 °C [15] | 618 |
virF-R | TGGGCTTGATATTCCGATAAGTC | |||
sigA | sigA-F | CCGACTTCTCACTTTCTCCCG | 58 °C [16] | 430 |
sigA-R | CCATCCAGCTGCATAGTGTTTG | |||
sepA | sepA-F | GCAGTGGAAATATGATGCGGC | 58 °C [17] | 794 |
sepA-R | TTGTTCAGATCGGAGAAGAACG | |||
pic | pic-F | ACTGGATCTTAAGGCTCAGGAT | 58 °C [17] | 572 |
pic-R | GACTTAATGTCACTGTTCAGCG | |||
sat | sat-F | TCAGAAGCTCAGCGAATCATTG | 59 °C [16] | 930 |
sat-R | CCATTATCACCAGTAAAACGCACC | |||
set1A | set1A-F | TCACGCTACCATCAAAGA | 57 °C [13] | 309 |
set1A-R | TATCCCCCTTTGGTGGTA | |||
set1B | set1B-F | GTGAACCTGCTGCCGATATC | 57 °C [13] | 147 |
set1B-R | ATTAGTGGATAAAAATGACG | |||
ipaBCD | ipaBCD-F | GCTATAGCAGTGACATGG | 59 °C [18] | 500 |
ipaBCD-R | ACGAGTTCGAAGCACTC | |||
sen | sen-F | ATGTGCCTGCTATTATTTAT | 55 °C [13] | 799 |
sen-R | CATAATAATAAGCGGTCAGC |