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Table 1 Primers and probes used to detect and sequence NoV GII

From: Molecular epidemiology of genogroup II norovirus infections in acute gastroenteritis patients during 2014–2016 in Pudong New Area, Shanghai, China

 

Primer/probe sequencea,b

Location of 5′ end

References

qRT-PCR

F

CARGARBCNATGTTYAGR TGGATGAG

5003

[13]

R

TCGACGCCATCTTCATTCACA

5100

P

TGGGAGGGCGATCGCAATCT

5048

RT-PCR

F

CNTGGGAGGGCGATCGCAA

5058

[14]

R

CCRCCNGCATRHCCRTTRTACAT

5401

  1. aMixed bases are as follows: Y, C or T; R, A or G; B, not A; N, any
  2. bF, R and P represent forward primer, reverse primer and oligonucleotide capture probe, respectively