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Table 1 General features, sources, malonate reaction, and genomic attributes of the strains used in this study

From: Genomic characterization of malonate positive Cronobacter sakazakii serotype O:2, sequence type 64 strains, isolated from clinical, food, and environment samples

Strain Source (isolation date) Malonate utilizeda Serotype Sequence type Clonal complex Draft genome size (bp) G+C content (%) Contigs CDS NCBI accession no.
7405-71-1A (Comp 11) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,432,172 56.74 124 4104 NHQL00000000
7405-68-2 (Comp 19) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,537,591 56.85 121 4205 NHQM00000000
7405-71-1B (Comp 20) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,564,009 56.77 190 4228 NEXY00000000
867009 DFI106-1 (Comp 45) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,731,392 56.8 117 4213 NEXZ00000000
867009 RF106-1 (Comp 46) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,728,640 56.77 107 4407 NEYA00000000
7405-80-2 (Comp 49) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,498,706 56.9 108 4159 NEYB00000000
7405-68-4 (Comp 53) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,545,852 56.8 101 4,177 NEYC00000000
7405-75-1 (Comp 54) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,540,274 57 135 4210 NEYD00000000
7405-75-2 (Comp 57) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,538,462 56.8 101 4200 NEYE00000000
0210ELJ 72-1 (Comp 59) Environmental, compliance activity (2014), USA (+) Csak O:2 64 64 4,649,928 56.5 173 4310 NEYF00000000
CDC 1121-73c Human, clinical Bronchial. wash (1973), USA (+) Csak O:2 64 64 4,443,589 57 238 4112 MCOD00000000
GK 1025c Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,599,266 56.7 94 4295 MCOE00000000
GK 1026 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,603,121 56.73 126 4298 NEYG00000000
GK 1027 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,603,249 56.7 34 4304 NHQN00000000
GK 1028 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 ND ND ND ND ND ND Not Yet Submitted
GK 1029 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,589,912 56.7 95 4292 NHQO00000000
GK 1030 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,601,082 56.7 37 4286 NHQP00000000
GK 1034 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,604,244 56.7 29 4296 NHQQ00000000
GK 1035 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,597,230 56.7 50 4282 NHQR00000000
GK 1326 Environmental, PIF manufacturing facility (2015), Germany (+) Csak O:2 64 64 4,423,544 56.73 153 4096 NEYH00000000
E772 Milk powder, France (+) Csak O:2 64 64 4,537,187 56.8 351 4163 NHQS00000000
H169/1/16 Environmental, PIF manufacturing facility (2012), Switz (+) Csak O:2 64 64 4,474,959 57 32 4139 NHTV00000000
Jor172 Food, spices, Jordan (+) Csak O:2 64 64 4,330,450 57 25 4000 NCWD00000000
Cro2819A3c Mushroom (2017), China ND ND 64 64 4,725, 000 57.2 168 3994 NZMBSC00000000
C. universalis NCTC9529T Water, (1956), UK (+) Cuni O:1 54 Unknownb 4,388,239 55.79 16 3977 CAKX00000000
C. condimenti LMG26250T Fermented spiced sausage, (2010), Slovakia (+) Unknown 98 Unknownb 4,480,620 55.8 155 4169 CAKW00000000
C. dublinensis LMG23823T Environmental, milk powder manufacturing plant (2004), Ireland (+) Cdub O:1 106 Unknownb 4,644,913 56.16 41 4172 CP012266
C. malonaticus LMG23826T Clinical, breast abscess (1977), USA (+) Cmal O:2 7 7 4,419,871 54.9 69 4041 CP013940
C. turicensis LMG23827T Clinical, blood (2005), Switzerland (+) Ctur O:1 19 24 4,599,092 57.2 1 4296 FN543093
C. muytjensii 51329T Unknown, USA (+) Cmuy O:2 81 81 4,355,922 56.17 32 3973 CP012268
C. sakazakii BAA-894 Infant formula (2001), USA (−) Csak O:1 1 1 4,530,777 56.7   4211 CP000783
  1. Cronobacter species identities were established using the species-specific rpoβ PCR assay as described by Stoop et al. [23] and Lehner et al. [24], and the cgcA species-specific PCR assay as described by Carter et al. [20]. Moreover, serotyping was assigned using the molecular-based serogrouping scheme described by Yan et al. [21], the results of which confirmed results of the rpoβ- and cgcA-based PCR species identification assays for each strain
  2. ND not determined
  3. aMalonate utilization was performed using Ewing Modified Malonate Broth according to the original assay described by Leifson [28] and modified by Ewing et al. [29]. Results were summarized after 48 h incubation at 37 °C
  4. bNo clonal complex was found for this strain in the Cronobacter MLST database
  5. cC. sakazakii strain Cro2819A3 was submitted to NCBI by Zeng et al. [27], and strains 1025 and 1121-73 were previously reported by Chase et al. [18]