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Fig. 5 | Gut Pathogens

Fig. 5

From: Rapid Isolation of intact Salmonella-containing vacuoles using paramagnetic nanoparticles

Fig. 5

Microscopic analysis of purified SCVs. Bacteria recovered by application of a magnetic field after lysis of THP-1 macrophages were incubated in presence of antibodies against the standard SCV markers Rab5 and LAMP-1. GFP-expressing Salmonella show exterior labelling with both Rab5 and LAMP-1 indicating intact, isolated SCVs. The bottom panel shows a close-up of an individual SCV showing a single bacterium (green), LAMP-1 staining (red)

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