Fig. 5
From: Rapid Isolation of intact Salmonella-containing vacuoles using paramagnetic nanoparticles
Microscopic analysis of purified SCVs. Bacteria recovered by application of a magnetic field after lysis of THP-1 macrophages were incubated in presence of antibodies against the standard SCV markers Rab5 and LAMP-1. GFP-expressing Salmonella show exterior labelling with both Rab5 and LAMP-1 indicating intact, isolated SCVs. The bottom panel shows a close-up of an individual SCV showing a single bacterium (green), LAMP-1 staining (red)