Table 5 Gene primers used in this study
Gene | Primer sequence (5′-3′) | PCR product (bp) | References |
|---|---|---|---|
alr | F: CTGGAAGAGGCTAGCCTGGACGAG R: AAAATCGCCACCGGTGGAGCGATC | 369 | [90] |
pic | F: GGGTATTGTCCGTTCCGAT R: ACAACGATACCGTCTCCCG | 1176 | [93] |
astA | F: CCATCAACACAGTATATCCGA R: GGTCGCGAGTGACGGCTTTGT | 111 | [73] |
aggR | F: ACGCAGAGTTGCCTGATAAAG R: AATACAGAATCGTCAGCATCAGC | 400 | [94] |
stx1 | F: CGATGTTACGGTTTGTTACTGTGACAGC R: AATGCCACGCTTCCCAGAATTG | 244 | [94] |
stx2 | F: GTTTTGACCATCTTCGTCTGATTATTGAG R: AGCGTAAGGCTTCTGCTGTGAC | 324 | [94] |
eae | F: TGAGCGGCTGGCATGAGTCATAC R: TCGATCCCCATCGTCACCAGAGG | 241 | [95] |
bfp | F: GACACCTCATTGCTGAAGTCG R: CCAGAACACCTCCGTTATGC | 324 | [94] |
ipaH | F: GTTCCTTGACCGCCTTTCCGATACCGTC R: AAAATCGCCACCGGTGGAGCGATC | 619 | [7] |
est | F: ATTTTTCTTTCTGTATTGTCTT R: CACCCGGTACAGGCAGGATT | 190 | [96] |
elt | F: GGCGACAGATTATACCGTGC R: CGGTCTCTATATTCCCTGTT | 450 | [96] |
daaD | F: TGAACGGGAGTATAAGGAAGATG R: GTCCGCCATCACATCAAAA | 444 | [97] |
daaE | F: GAACGTTGGTTAATGTGGGGTAA R: TATTCACCGGTCGGTTATCAGT | 542 | [8] |
fimC | F: GGGTAGAAAATGCCGATGGTG R: CGTCATTTTGGGGGTAAGTG | 477 | [98] |
fimH | F: CGAGTTATTACCCTGTTTGCTG R: ACGCCAATAATCGATTGCAC | 878 | [73] |
aggA | F: GCTAACGCTGCGTTAGAAAGACC R: GGAGTATCATTCTATATTCGCC | 421 | [73] |
aafA | F: ATGTATTTTTAGAGGTTGAC R: TATTATATTGTCACAAGCTC | 518 | [20] |
agg3A | F: GTATCATTGCGAGTCTGGTATTCAG R: GGGCTGTTATAGAGTAACTTCCAG | 462 | [73] |
agg4A | F: TGAGTTGTGGGGCTAYCTGGACACC R: ATAAGCCGCCAAATAAGC | 169 | [74] |
lpfA | F: AGGCGGTGCATTCACTCTGGCATCT R: CCGCGTCGATAGCGGTATAGGCAGA | 446 | [99] |
sfa | F: CTCCGGAGAACTGGGTGCATCTTAC R: CGGAGGAGTAATTACAAACCTGGCA | 408 | [73] |
pap | F: GACGGCTGTACTGCAGGGTGTGGCG R: ATATCCTTTCTGCAGGGATGCAATA | 328 | [73] |
irp2 | F: AAGGATTCGCTGTTACCGGAC R: TCGTCGGGCAGCGTTTCTTCT | 264 | [100] |
fyuA | F: TGATTAACCCCGCGACGGGAA R: CGCAGTAGGCACGATGTTGTA | 785 | [34] |
escJ | F: CACTAAGCTCGATATATAGAACCC R: GTCAATGTTGATGTCGTATCTAAG | 824 | [80] |
escN | F: CGCCTTTTACAAGATAGAAC R: CATCAAGAATAGAGCGGAC | 854 | [101] |
escV | F: GATGACATCATGAATAAACTC R: GCCTTCATATCTGGTAGAC | 2128 | [80] |
espP | F: AAACAGCAGGCACTTGAACG R: GGAGTCGTCAGTCAGTAGAT | 1830 | [93] |
nleB | F: GGAAGTTTGTTTACAGAGACG R: AAAATGCCGCTTGATACC | 297 | [43] |
nleE | F: GTATAACCAGAGGAGTAGC R: GATCTTACAACAAATGTCC | 260 | [43] |
ent/espL2 | F: GAATAACAATCACTCCTCACC R: TTACAGTGCCCGATTACG | 233 | [43] |
cnf1 | F: GGCGACAAATGCAGTATTGCTTGG F: GACGTTGGTTGCGGTAATTTTGGG | 552 | [93] |
cnf2 | F: GTGAGGCTCAACGAGATTATGCACTG R: CCACGCTTCTTCTTCAGTTGTTCCTC | 839 | [93] |
cdt-I | F: CAATAGTCGCCCACAGGA R: ATAATCAAGAACACCACCAC | 412 | [102] |
cdt-II | F: GAAAGTAAATGGAATATAAATGTCCG R: TTTGTGTTGCCGCCGCTGGTGAAA | 556 | [102] |
invE | F:CGATCAAGAATCCCTAACAGAAGAATCAC R: CGATAGATGGCGAGAAATTATATCCCG | 766 | [94] |
hlyA | F: GCATCATCAAGCGTACGTTCC R: AATGAGCCAAGCTGGTTAAGCT | 533 | [100] |
pet | F: TTTCCAGCACTTCCTGTTCC R: ATTTCCAACGTCTACGCCAT | 297 | [103] |
sat | F: GCAGCAAATATTGATATATCA R: GTTGTTGACCTCAGCAAGGAA | 2913 | [80] |
subAB | F: TATGGCTTCCCTCATTGCC R: TATAGCTGTTGCTTCTGACG | 556 | [104] |