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Fig. 1 | Gut Pathogens

Fig. 1

From: Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Fig. 1

DP-HMGS was optimized for robust detection of DPs in a multiplex reaction. a Before optimization of the DP-HMGS assay, eighteen targets (from left to right: hum_RNA, S. typhimurium, S. enteritidis, EIEC, Shigella, HADV, EHEC, HASV, ETEC, Vibrio, C. difficile, C. jejuni, norovirus, hum_DNA, EPEC, EAEC, rotavirus, IC) exhibited relatively large differences in signal intensity. Notably, the specific peaks for Y. enterocolitica and E. coli O157 were not detected by the DP-HMGS assay prior to optimization. b After optimization, all 20 targets (from left to right: hum_RNA, S. typhimurium, S. enteritidis, EIEC, Shigella, HADV, EHEC, HASV, Y. enterocolitica, ETEC, Vibrio, C. difficile, C. jejuni, E. coli O157, norovirus, hum_DNA, EPEC, EAEC, rotavirus, IC) reached intermediate signal levels. Notably, the specific peaks for Y. enterocolitica and E. coli O157 were now visible at 165 bp and 218 bp

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Gut Pathogens

ISSN: 1757-4749