Skip to main content
Fig. 5 | Gut Pathogens

Fig. 5

From: Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Fig. 5

The uniformly high sensitivity of DP-HMGS corrected previous conclusions regarding the major causes of infectious diarrhoeas. a Comparisons of culture-based methods versus DP-HMGS with regard to sequencing-based detection of 13 diarrhoeal bacterial pathogens were conducted. The sensitivity of DP-HMGS for the detection of Vibrio (P = 0.000), S. typhimurium (P = 0.000), Shigella (P = 0.005), C. difficile (P = 0.000), C. jejuni (P = 0.000), EPEC (P = 0.000), ETEC (P = 0.000), EIEC (P = 0.003) and E. coli O157 (P = 0.008) was significantly lower than that of culture-based detection. Notably, the sensitivity of DP-HMGS was at least twice that of culture-based methods for most bacterial species and as virtually identical to that of sequencing. b Uniform sensitivity of DP-HMGS in detecting both bacterial and viral DPs corrected the ratios of bacterial versus viral causes of diarrhoeas, demonstrating the overwhelming importance of bacterial infections. c The epidemiological contribution of individual DP species was ranked based on the frequency of positive detection rates observed using conventional methods (top) and DP-HMGS (bottom). Notably, DP-HMGS corrected the epidemiological outcomes attained by combining the results of conventional methods, which were profoundly skewed by the low and highly variable sensitivity of culture-based methods in detecting bacterial DPs

Back to article page