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Table 2 DP-HMGS exhibited uniformly high sensitivity of DP detection compared to the sequencing method

From: Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Pathogens HMGS Sequencing Sensitivity Specificity PPV NPV Accuracy
+
Vibrio + 39 1 0.975 0.998 0.975 0.998 0.997
1 572
S. typhimurium + 41 1 1.000 0.998 0.976 1.000 0.998
0 571
S. enteritis + 4 2 0.667 0.997 0.667 0.997 0.993
2 605
Shigella + 9 1 1.000 0.998 0.900 1.000 0.998
0 603
C. difficile + 28 0 1.000 1.000 1.000 1.000 1.000
0 585
C. jejuni + 44 0 0.978 1.000 1.000 0.998 0.998
1 568
Y. enterocolitica + 1 0 1.000 1.000 1.000 1.000 1.000
0 612
EPEC + 151 0 1.000 1.000 1.000 1.000 1.000
0 462
ETEC + 29 2 1.000 0.997 0.935 1.000 0.997
0 582
EAEC + 27 0 1.000 1.000 1.000 1.000 1.000
0 586
EIEC + 8 1 1.000 0.998 0.889 1.000 0.998
0 604
EHEC + 4 0 1.000 1.000 1.000 1.000 1.000
0 609
E. coli O157 + 6 2 1.000 0.997 0.750 1.000 0.997
0 605
Norovirus + 31 7 1.000 0.988 0.816 1.000 0.982
0 575
Rotavirus + 55 2 1.000 0.996 0.964 1.000 0.990
0 556
Adenovirus + 9 3 1.000 0.995 0.750 1.000 0.993
0 601
Astrovirus + 4 0 1.000 1.000 1.000 1.000 0.997
0 609
  1. The outcomes of DP-HMGS and sequencing-based DP detection methods were compared. Sensitivity = TP/(TP + FN) × 100, Specificity = TN/(TN + FP) × 100; PPV and NPV were calculated as follows: PPV = TP/P, NPV = TN/N. Notably, the sensitivity of DP-HMGS is high and comparable to that of sequencing for virtually all DP species
  2. DP-HMGS diarrheal pathogens high-throughput multiple genetic detection system, PPV positive predict value, NPV negative predict value