Fig. 5

Phylogenetic relationship of all 28 spacer sequences detected in 16 CRISPR-positive V. parahaemolyticus isolates. The tree was constructed using maximum likelihood method. Numbers at branch-points represent confidence values obtained after bootstrap analysis of the maximum likelihood tree using 1000 replicates. The scale bar represents 0.1 substitutions per nucleotide position. PSU5256-s2, spacer no. 2 of V. parahaemolyticus PSU5256; C1, tdh⁺ trh1⁺; C2, tdh⁺ trh2⁺; C3, tdh⁻ trh1⁺; C4, tdh⁻ trh2⁺