Fig. 1

Specificity and sensitivity of the IS900 nPCR in DNA extracts from the modified DNAzol^® and the standard phenol/chloroform/isoamyl-alcohol DNA extraction protocols. nPCR based on the IS900 specific to MAP was performed on DNA template extracted by the standard phenol/chloroform/isoamyl-alcohol DNA extraction (I) and the modified DNAzol^® DNA extraction technique (II). A 298 bp fragment on 2% agarose gel is positive for MAP. a (1) Non-pathogenic E. coli strain K-12; (2) S. aureus; (3) L. monocytogenes; (4) K. pneumoniae; (5) M. smegmatis; (6) M. avium subspecies avium; (7) M. xenopi; (8) M. fortuitum subspecies fortuitum; (9) MAP Clinical Strain JF7. b (1) MAP Strain 1; (2) MAP Strain 3; (3) MAP Strain 8B; (4) MAP Para 18; (5) MAP UCF3; (6) MAP UCF5; (7) MAP UCF7; (8) MAP Linda; (9) MAP MS137. c Serial dilution of MAP UCF4 DNA concentrations were analyzed by nPCR. (1) 31.7 ng/μL; (2) 3.17 ng/μL; (3) 317 pg/μL; (4) 31.7 pg/μL; (5) 3.17 pg/μL; (6) 317 fg/μL; (7) 31.7 fg/μL; (8) 3.17 fg/μL; (9) 317 ag/μL; (10) 31.7 ag/μL. +: MAP UCF4; N: No DNA; M: molecular weight marker