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Fig. 4 | Gut Pathogens

Fig. 4

From: Development of multiplex PCR and multi-color fluorescent in situ hybridization (m-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

Fig. 4

Gram stain, acid-fast stain and m-FISH detection of bacterial cultures. Gram stain (1), acid-fast stain (2), and oligonucleotide m-FISH (3) images of bacterial cultures: a, b Non-pathogenic E. coli strain K-12; c MAP UCF4; and d K. pneumoniae. For -m-FISH detection: a3 m-FISH using non-pathogenic E. coli K-12 18s probe labeled with AF647 fluorophore; b3 m-FISH using AIEC strain LF82 gipA probe labeled with AF568 fluorophore; c3 m-FISH using MAP UCF4 IS900 AV1 probe labeled with AF488 fluorophore; and d3 m-FISH using K. pneumoniae 23s probe labeled with AF546 fluorophore. All microscopic images were obtained at 1000 × magnification. White measurement bar found in D3 represents 100 μm. All m-FISH images were obtained using CSLM

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Gut Pathogens

ISSN: 1757-4749