Fig. 1

Infection of Caco-2 cells with C. jejuni disturbs the occludin patterns in a htrA-dependent manner. α-Occludin (green) and α-C. jejuni immunostaining of Caco-2 mock control cells (a) or cells infected with wild-type (wt) strain 81–176 (b), ΔhtrA knockout mutant (c) or ΔhtrA complemented with wild-type htrA (d). The inlay in panel A shows the belt-like pattern of occludin around the polarized cells as expected (blue arrows). Infection was performed for 12 h at an MOI of 100. The pictures revealed a redistribution of upon wt infection but not ΔhtrA mutant (white arrows). Co-localization of occludin with C. jejuni was not observed. DAPI staining (blue) was used for visualization of the DNA in the nuclei