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Table 1 Bacterial strains and plasmids

From: Campylobacter jejuni enters gut epithelial cells and impairs intestinal barrier function through cleavage of occludin by serine protease HtrA

Strain/plasmid Genotype References
C. jejuni 81–176 Wild-type [11]
C. jejuni 81–176 ΔhtrA C. jejuni strain 81–176 with ΔhtrA deletion, CatR [11]
C. jejuni 81–176 ΔhtrA/htrA ΔhtrA deletion complemented with wild-type htrA from C. jejuni 81–176, KanR [39]
E. coli BL21(DE3)pLysS F–, ompT, hsdSB (rB–, mB–), dcm, gal, λ(DE3), pLysS, CatR Promega
pKB1004 pET28a, wt htrA from the C. jejuni NCTC11168 strain, C-terminal 6× His tag, KanR [69]
pUZCj4 pET26b, wt htrA from the C. jejuni NCTC11168 strain, C-terminal 6× His tag, KanR This work