Table 1 Bacterial strains and plasmids

C. jejuni 81–176

Wild-type

[11]

C. jejuni 81–176 ΔhtrA

C. jejuni strain 81–176 with ΔhtrA deletion, Cat^R

[11]

C. jejuni 81–176 ΔhtrA/htrA

ΔhtrA deletion complemented with wild-type htrA from C. jejuni 81–176, Kan^R

[39]

E. coli BL21(DE3)pLysS

F–, ompT, hsdS_B (r_B–, m_B–), dcm, gal, λ(DE3), pLysS, Cat^R

Promega

pKB1004

pET28a, wt htrA from the C. jejuni NCTC11168 strain, C-terminal 6× His tag, Kan^R

[69]

pUZCj4

pET26b, wt htrA from the C. jejuni NCTC11168 strain, C-terminal 6× His tag, Kan^R

This work