Fig. 1

Colonic inflammation induced by 1,25(OH)₂D₃ deficiency. a Representative images and histological scores of colon sections from 8 to 10-month wild type and Cyp27b1^−/− mice (n = 5). Magnification, ×100, ×200, ×400. b Spleen weights of 8–10-month wild type and Cyp27b1^−/− mice. c Real-time RT-PCR analysis for the gene expression of IL-1ɑ, IL-1β, IL-6, HGF1, and MMP-3 on the extracts of colon from 8 to 10-month wild type and Cyp27b1^−/− mice. Data represent mean ± S.E.M from three independent experiments. *P < 0.05, **P < 0.01, WT: wild type, KO: Cyp27b1^−/−