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Fig. 3 | Gut Pathogens

Fig. 3

From: 1,25(OH)2D3 deficiency-induced gut microbial dysbiosis degrades the colonic mucus barrier in Cyp27b1 knockout mouse model

Fig. 3

Deficiency in 1,25(OH)2D3 leads to degradation of the colonic mucus barrier. a Alcian blue-stained colonic sections showing the mucus layer (arrows). The histogram showing the mean percentage of the mucus layer. Scale bars, 100 μm. b Representative images showing Alcian blue staining of colonic crypts from 8 to 10-month wild type and Cyp27b1/ mice. The histogram showing the mean number of goblet cell density in colonic crypts. Original magnification, ×400. c Real-time RT-PCR analysis of the gene expression of Muc1, Muc2, Muc3 and Muc4 on extracts of colon from 8 to 10-month wild type and Cyp27b1/ mice for. d Relative levels of total bacteria in mesenteric lymph nodes (MLNs) from 8 to 10-month wild type and Cyp27b1/ mice. *P < 0.05, **P < 0.01. WT: wild type, KO: Cyp27b1/

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