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Fig. 4 | Gut Pathogens

Fig. 4

From: 1,25(OH)2D3 deficiency-induced gut microbial dysbiosis degrades the colonic mucus barrier in Cyp27b1 knockout mouse model

Fig. 4

1,25(OH)2D3 affects A. muciniphila colonization in gut. a Relative abundance of A. muciniphila in feces and b Relative levels of total bacteria in MLNs from wild type and Cyp27b1/ mice with 10–12-week age. c The mucus layer (arrows) from wild type and Cyp27b1/ mice with the age of 10–12 weeks assessed on Alcian blue-stained colonic sections. The histogram showing the mean percentage of the mucus layer. Scale bars, 100 μm. d Relative abundance of A. muciniphila in feces from wild type, Cyp27b1/ and Cyp27b1/ mice feed with 1,25(OH)2D3 at the age of 8–10 months. e The mucus layer (arrows) from wild type, Cyp27b1/ and Cyp27b1/ mice feed with 1,25(OH)2D3 on Alcian blue-stained colonic sections. The histogram showing the mean percentage of the mucus layer. Scale bars, 100 μm. f CD3 and F4/80 expression in colon tissues from the wild type and Cyp27b1/ mice at the age of 8–10 months by immunohistochemical staining. Magnification, ×400. The histogram showing the mean percentage of the CD3 or F4/80 positive cells from five randomly selected fields. *P < 0.05, **P < 0.01. WT: wild type, KO: Cyp27b1/, KO + VD: Cyp27b1/ mice feed with 1,25(OH)2D3

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